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. 2013 Nov 13;8(11):e78766. doi: 10.1371/journal.pone.0078766

Figure 6. 280B is over-expressed in prostate tumors and correlates with reduced p53 protein expression.

Figure 6

(A)Real-time PCR and Western blotting were used to detect the expression of 280B in normal prostate (PrEC) and prostate cancer cell lines (LNCaP, C81, and CWR-22Rv1). (B) Immuno-staining of 280B in 4 matched pairs of prostate tissues, normal versus tumor. (C) Cell extract from 4 normal prostate tissues and 9 tumor tissues were subjected to Western blotting to detect 280B, p53, and Mdm2 expression levels. β-actin served as a loading control.