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. 2013 Nov 13;8(11):e78885. doi: 10.1371/journal.pone.0078885

Figure 9. Functional activity of selected targets.

Figure 9

(A) mRNA knockdown of B7H3 inhibits proliferation in HUVECs in a titration dependent manner. A representative screen and a titration experiment with duplexes 2 and 3 in HUVECs are shown. Proliferation was monitored by using the Alamar Blue assay, and SPA [3H]. Thymidine uptake assay system. (B) mRNA knockdown of ATP1B3 inhibits proliferation and increases apoptosis in HMVECs in a titration dependent manner. A titration experiment with duplexes 2 in HMVECs is shown. Proliferation was monitored by using the Alamar Blue assay, and apoptosis was measured by caspase 3/7 activity.