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. 2013 Nov 13;8(11):e78966. doi: 10.1371/journal.pone.0078966

Figure 1. Generation and validation of BCG ΔureC::hly_hIL7 and BCG ΔureC::hly_hIL18.

Figure 1

A. Organisation of the cytokine expression cassette for stable transformation of BCG ΔureC::hly. B. Confirmation of hIL-7 and hIL-18 gene presence (gDNA as template) and transcription (cDNA as template) in BCG ΔureC:hly_hIL7 or BCG ΔureC:hly_hIL18, respectively using PCR or RT-PCR. C. Confirmation of hIL-18 and hIL-7 protein expression by the respective strains using ELISA. Experiments were performed twice with similar outcome.