Figure 4. BCG ΔureC::hly_hIL7 and BCG ΔureC::hly_hIL18 enhance antigen-specific Th1 responses.
Flow cytometric analysis of CD40L-expressing antigen-specific CD4+ T cell responses (A) and cytokine-producing CD40L+CD4+ T cells (B) in the lungs, spleen and draining (inguinal) lymph nodes (dLNs) of vaccinated mice following in vitro re-stimulation with M. tuberculosis H37Rv whole cell lysate. Only comparisons made with BCG ΔureC::hly are displayed. Shown are means ± SEM (n = 3 mice per group) analyzed using two-way ANOVA and Tukey’s post-hoc test; *p<0.05. Experiment was performed twice.