Table 3. Grading of spleen amyloid and quantification of RPMs after macrophage depletion.

Group	Treatment	Sacrificed day	No. of mice/no. of mice with amyloid	Amyloid grade	% of RPMs±SD
A	Untreated	0, 4, 16	12/0	0	100
H	CL	0	3/0	0	0±0***
J	CL	4	10/0	0	40±19***
K	CL+AEF	4	15/0	0	54±18***
L	CL+AgNO3	4	5/0	0	36±10***
M	CL+AEF+AgNO3	4	17/9	1+	52±15***
N	CL+AEF	16	6/1	2+	99±6
O	CL+AgNO3	16	7/7	2+	118±1*
P	CL+AEF+AgNO3	16	4/4	2+−4+	107±3

See figure 1 for definition of groups, *p<0.05; *** p≤0.001.

Presence of spleen amyloid was analysed after Congo red staining and RPMs were detected with antibodies specific for F4/80, CL = Clodronate containing liposomes.