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(A) Representative immunoblots of hepatic GP protein in Alaskan and Ohioan unfrozen (U), 48 h-frozen (F) and 120 h-thawed (T) frogs (2 samples per treatment). (B) Hepatic GP protein amounts (mean ± SEM; N = 4–8) during freezing (48 h) and thawing (120 h) in Alaskan and Ohioan R. sylvatica as compared to that in unfrozen frogs (U). Dagger indicates that the value differs between populations (Bonferroni, P<0.05).
