Table 1. Percentage of enzyme present as PKAc and total PKA activity during freezing and thawing in R. sylvatica.
| Alaskan | Ohioan | ||||
| Treatment | Percentage of PKAc | Total PKA activity | Percentage of PKAc | Total PKA activity | |
| Unfrozen | 25.5±3.1 | 1.03±0.14 | 28.8±1.4 | 0.87±0.05 | |
| Frozen | 2 h | 24.9±4.2 | 1.08±0.14 | 39.1±5.4† | 0.67±0.09 |
| 6 h | 31.1±4.7 | 1.06±0.13 | 31.2±3.1 | 0.74±0.12 | |
| 30 h | 17.7±2.7 | 1.02±0.11 | 6.4±1.6* | 0.48±0.06† | |
| 48 h | 15.6±1.7 | 0.80±0.10 | 12.4±2.4* | 0.65±0.09 | |
| Thawed | 6 h | 10.3±2.1* | 1.03±0.13 | 11.2±3.4* | 0.62±0.09 |
| 120 h | 22.0±3.9 | 1.08±0.17 | 32.1±6.6 | 0.66±0.05 | |
Values are mean ± SEM (N = 4–8). Total activity of PKA is in U mg−1 protein. Asterisk indicates that the value differs from the mean for the corresponding sample of unfrozen frogs (Dunnett’s, P<0.05); dagger indicates that the value differs between populations (Bonferroni, P<0.05).