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. 2013 Oct;11(4):541–547. doi: 10.2450/2013.0294-12

Figure 1.

Figure 1

GAT with granulocytes homozygous for HNA-3a, re-suspended in PBS or PBS containing 0.5% of either BSA I, BSA II, milk powder, or blocking reagent® and incubated with the anti-HNA-3a IgG fraction or a control IgG fraction. The granulocytes aggregated after incubation with anti-HNA-3a IgG. Non-specific aggregation due to the protein additives could be excluded, since no aggregation was observed when cells were incubated with control IgG. These results are representative of experiments with granulocytes from six different donors.