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Binding of anti-HNA-3a IgG (dark grey) or control IgG (grey) to PMN after re-suspension in different solutions. No difference could be observed after re-suspension of the PMN in BSA compared to re-suspension in buffer. In contrast, re-suspension in milk powder or blocking reagent® resulted in stronger or weaker binding of anti-HNA-3a IgG to CTL2 compared to non-treated PMN. These results are representative of experiments with granulocytes from three different donors.
