Figure 3.

GAT with HNA-3a homozygous granulocytes resuspended in PBS which were incubated with either anti-HNA-3a IgG or control IgG. Prior to the GAT, Terasaki plates were blocked with 0.5% of either BSA I, BSA II, milk powder, Tween^®20 or blocking reagent^®. Control plates were incubated with PBS only. Using blocked Terasaki plates, granulocytes showed typical aggregation patterns after incubation with anti-HNA-3a antibodies, while they failed to aggregate when unblocked plates were used. Non-specific aggregation due to plate blocking could be excluded, since no aggregation was observed when cells were incubated with control IgG. This figure shows a representative GAT result for granulocytes from six different donors.