Table I.

Relevance of re-suspension buffer.

	Granulocyte re-suspension buffer
	Plasma	PBS
Anti-HNA-3a IgG	+	−
Anti-HNA-3a plasma	+	+

Legend

+: ≥10 % aggregation; −: no aggregation.

GAT reactivity of granulocytes homozygous for HNA-3a re-suspended in either plasma or PBS and incubated with HNA-3a plasma or its corresponding IgG fraction. Using this original GAT setting, granulocytes re-suspended in PBS aggregated in the presence of anti-HNA-3a plasma, but not in the presence of the respective IgG fraction. These results are representative of experiments with granulocytes from ten different donors.