Table II.

Impact of BSA in the IgG dilution buffer.

Anti-HNA-3a IgG dilution	Granulocyte re-suspension buffer
	PBS	PBS with 0.5% BSA
in PBS	−	+
in PBS with 0.5 % BSA	−	+

Legend

+: ≥10 % aggregation; −: no aggregation.

GAT reactivity of HNA-3a homozygous granulocytes re-suspended in PBS or PBS containing 0.5% BSA, which were incubated with two HNA-3a IgG dilutions (1:4) in PBS or PBS containing 0.5% BSA. Without protective agents in the re-suspension buffer, granulocytes did not aggregate when incubated with anti-HNA-3a IgG. However, when granulocytes were re-suspended in 0.5% BSA prior to the GAT, aggregation occurred with both anti-HNA-3a IgG dilutions (n =3).