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. 2013 Nov 13;8(11):e80435. doi: 10.1371/journal.pone.0080435

Figure 4. Endolysin lytic activity and binding assay.

Figure 4

A. The OD600 descent is used to evaluate the lytic activity of IME-EF1 endolysin on the isolated E. faecalis, E. faecium, and S. aureus. B. Assay of IME-EF1 endolysin binding to sensitive bacterial strains. The expressed IME-EF1 endolysin was incubated with sensitive bacteria E. faecalis 002 and E. faecium 010, as well as the insensitive bacteria E. faecium 81 and S. aureus for 10 min, and the bacteria was then pelleted and the remaining supernatant endolysin was measured with ELISA at OD490. “Input” means no bacteria were added. Significance was determined using independent t test (* P<0.05).