Table 2. Determination of optimal multiplicity of infection.

Tube No.	Number of bacteria	Number of bacteriophage	M.O.I	Titer at 6h(PFU/ml)
1	1.0×106	1.0×104	0.01	5.0×107
2	1.0×106	1.0×105	0.1	1.9×108
3	1.0×106	1.0×106	1	8.3×109
4	1.0×106	1.0×107	10	3.9×109
5	1.0×106	1.0×108	100	1.4×109

M.O.I Multiplicity of Infection

E. faecalis 002 was infected with bacteriophage IME-EF1 at different M.O.I. The phage multiplication was calculated at 6 h post infection, and indicated with plaque forming unit (PFU) per milliliter culture.