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. 2013 Nov 13;8(11):e79770. doi: 10.1371/journal.pone.0079770

Figure 2. Role of BtaF in the adherence of B. suis to an abiotic surface and ECM components.

Figure 2

Expression of BtaF was evaluated in E. coli (A) and B. suis (B) strains by Western blot analysis. Total cell proteins and secreted proteins were separated on a semi-native gel (10%) and transferred to PDVF membrane. Binding to the wells of PE microtiter plates (C and D) or to immobilized ligands (E and F) of E. coli pBBRbtaF and E. coli pBBR1MCS (C and E), B. suis wild type, B. suis ΔbtaF and B. suis ΔbtaF pBBRbtaF (D and F) was assayed. Values correspond to the percentage of attachment to the PE surface or percentage of binding to ECM components. A value of 100% was assigned to control strains. Data represent the means ± and standard deviations (SD) of the results of a representative experiment done in triplicate. Three independent experiments were performed with similar results. Data were analyzed by Student's t test and by one-way ANOVA followed by a Tukey a posteriori test. *, significantly different from control (P<0.05), with 95% confidence.