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. 2013 Nov 13;8(11):e80098. doi: 10.1371/journal.pone.0080098

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Cftr mRNA expression analysis of calvarial osteoblasts, undifferentiated and osteoclast-differentiated (M-CSF/RANKL-treated) RAW264.7 murine monocyte cells. Trap mRNA expression served as a control for osteoclast formation. (B) Immunofluorescence did not reveal CFTR (red) expression in murine bone marrow-derived multinucleated osteoclasts. Membrane-localized protein Na⁺/H⁺ exchange regulatory cofactor-1 (NHERF1) (green) served as a positive control for membrane staining. Nuclei (blue) was identified by DAPI staining.