Table 1. Primers information and RT-qPCR conditions used in the trial.
| Gene | NCBI accession number | Oligo sequence (5'→3') |
Amplicon length | Annealing T | |
|---|---|---|---|---|---|
| TLR2 | NM_213761.1 | Forward | GCGGAAGATAATGAACACCA | 150 bp | 62°C |
| Reverse | AGAGCGATAAAGACCAGCAT | ||||
| TLR3 | NM_001097444.1 | Forward | CAAAACCAGCAACACGACTT | 110 pb | 59°C |
| Reverse | GAAGCCAAGCAAAGGAATCA | ||||
| TLR4 | NM_001113039.1 | Forward | CTTCCTCCTGGTATCTGTGG | 113 bp | 60°C |
| Reverse | CAAAGGCGTCATAGGTGCTT | ||||
| TLR5 | NM_001123202.1 | Forward | CAGCGACCAAAACAGATTGA | 122 pb | 59°C |
| Reverse | TGCTCACCAGACAGACAACC | ||||
| PIGR | NM_214159.1 | Forward | AGCCAACCTCACCAACTTCC | 105 bp | 62°C |
| Reverse | CTGCTAATGCCCAGACCAC | ||||
| HMBS2 | DQ845174 | Forward | AGGATGGGCAACTCTACCTG | 83 bp | 62°C |
| Reverse | GATGGTGGCCTGCATAGTCT | ||||
| RPL4 | DQ845176 | Forward | CAAGAGTAACTACAACCTTC | 122 bp | 60°C |
| Reverse | GAACTCTACGATGAATCTTC | ||||
TLR2, toll-like receptor 2; TLR3, toll-like receptor 3; TLR4, toll-like receptor 4; TLR5, toll-like receptor 5; PIGR, Polymeric immunoglobulin receptor; HMBS2, Hydroxymethylbilane synthase 2; RPL4, Ribosomal protein L4.