Figure 6. Flanking gypsy insulators promote enhancer-promoter communication in trans.

QPCR analysis (top graph) and immunostaining (bottom panels) of wing discs from all seven 2-insulator genotypes. For microscopy, all images were normalized to P⁺E⁺/P⁺E⁺ and minimum/maximum level corrections were applied equally to all genotypes using ImageJ and false-colored green. Error bars represent standard error of the mean (S.E.M) and scalebars are 50 μm.