Table 2.
Proteins showing differential responses
| Spot No. | IPI No. | Gene Name | Protein Name | Mascot Score | Fold Change (Healed/ Dehisced) | T-test | Th. MW | Th. pI |
|---|---|---|---|---|---|---|---|---|
| 59 |
IPI00440484 |
PER1 |
Period Circadian protein homolog 1 |
133 |
-1.23 |
0.0414 |
137667 |
5.73 |
| 95* |
IPI00645206 |
PCDH17 |
Isoform 1 of Protocadherin 17 |
131 |
-1.22 |
0.0121 |
127405 |
5.03 |
| 99* |
IPI00235407 |
STK36 |
Isoform 1 of Serine/threonine protein kinase 3 |
142 |
-1.24 |
0.0018 |
145728 |
5.57 |
| 102 |
IPI00185661 |
USP32 |
Ubiquitin carboxyl terminal hydrolase 32 |
125 |
-1.2 |
0.0164 |
145671 |
6.18 |
| 106 |
IPI00470744 |
PARG |
Isoform 2 of Poly (ADP-ribose) glycohydrolase |
113 |
-1.22 |
0.0106 |
103503 |
5.58 |
| 168* |
IPI00290328 |
PTPRJ |
Receptor type tyrosine protein phosphotase precursor |
130 |
-1.51 |
0.0061 |
152960 |
5.8 |
| 195* |
IPI00017601 |
CP |
Ceruloplasmin |
101 |
-1.27 |
0.0116 |
122983 |
5.44 |
| 198* |
IPI00017601 |
CP |
Ceruloplasmin |
121 |
-1.39 |
0.0076 |
122983 |
5.44 |
| 199 |
IPI00017601 |
CP |
Ceruloplasmin |
182 |
-1.31 |
0.0095 |
122983 |
5.44 |
| 200 |
IPI00017601 |
CP |
Ceruloplasmin |
116 |
-1.33 |
0.0075 |
122983 |
5.44 |
| 201 |
IPI00017601 |
CP |
Ceruloplasmin |
112 |
-1.3 |
0.0254 |
122983 |
5.44 |
| 202 |
IPI00017601 |
CP |
Ceruloplasmin |
154 |
-1.31 |
0.0130 |
122983 |
5.44 |
| 329 |
IPI00011736 |
PIK3R2 |
Phosphatidylinositol 3 kinase regulatory subunit 2 |
88 |
1.26 |
0.0259 |
81837 |
6.03 |
| 330 |
IPI00783987 |
C3 |
Complement C3 |
115 |
1.55 |
0.0003 |
188569 |
6.02 |
| 333 |
IPI00011736 |
PIK3R2 |
Phosphatidylinositol 3 kinase regulatory subunit 2 |
111 |
1.25 |
0.0046 |
81837 |
6.03 |
| 337 |
IPI00783987 |
C3 |
Complement C3 |
88 |
1.53 |
0.0104 |
188569 |
6.02 |
| 341* |
IPI00783987 |
C3 |
Complement C3 |
147 |
1.56 |
0.0006 |
188569 |
6.02 |
| 343* |
IPI00783987 |
C3 |
Complement C3 |
189 |
1.62 |
0.0006 |
188569 |
6.02 |
| 344 |
IPI00783987 |
C3 |
Complement C3 |
120 |
1.69 |
0.0048 |
188569 |
6.02 |
| 345 |
IPI00783987 |
C3 |
Complement C3 |
79 |
1.59 |
0.0125 |
188569 |
6.02 |
| 347 |
IPI00783987 |
C3 |
Complement C3 |
104 |
1.52 |
0.0339 |
188569 |
6.02 |
| 375* |
IPI00607814 |
XPNPEP1 |
Xaa-Pro aminopeptidase 1 Isoform 2 |
121 |
1.23 |
0.0415 |
72746 |
5.67 |
| 389 |
IPI00157535 |
EPS8L1 |
Isoform 4 of Epigrowth factor receptor kinase subunit 8 |
118 |
1.23 |
0.0168 |
88920 |
6.49 |
| 436 |
IPI00009089 |
FRS3 |
Fibroblast growth factor receptor substrate 3 |
121 |
-1.28 |
0.0198 |
55169 |
6.81 |
| 582 |
IPI00301255 |
IGSF21 |
Immunoglobulin superfamily member 21 |
109 |
-1.23 |
0.0480 |
51855 |
6.48 |
| 602 |
IPI00329555 |
F7 |
Isoform A of Coagulation factor VII |
178 |
-1.54 |
0.0232 |
53043 |
6.91 |
| 607 |
IPI00329555 |
F7 |
Isoform A of Coagulation factor VII |
130 |
-1.37 |
0.0462 |
53043 |
6.91 |
| 787 |
IPI00021891 |
FGG |
Fibrinogen gamma chain 1 |
135 |
1.33 |
0.0267 |
51511 |
5.37 |
| 794 |
IPI00847635 |
SERPINA3 |
Isoform 1 of alpha-1-antichymotrypsin |
146 |
1.57 |
0.0067 |
47792 |
5.33 |
| 800 |
IPI00847635 |
SERPINA3 |
Isoform 1 of alpha-1-antichymotrypsin |
216 |
1.22 |
0.0273 |
47792 |
5.33 |
| 814 |
IPI00219330 |
ILF3 |
Isoform 5 of Interleukin enhancer binding factor 3 |
252 |
-1.35 |
0.0205 |
74959 |
8.4 |
| 869 |
IPI00783625 |
SERPINB5 |
Isoform 1 of Serpin B5 |
120 |
1.27 |
0.0356 |
42530 |
5.72 |
| 872* |
IPI00847635 |
SERPINA3 |
Isoform 1 of alpha-1-antichymotrypsin |
111 |
1.39 |
0.0456 |
47792 |
5.33 |
| 876 |
IPI00847635 |
SERPINA3 |
Isoform 1 of alpha-1-antichymotrypsin |
283 |
1.24 |
0.0424 |
47792 |
5.33 |
| 976 |
IPI00004657 |
HLA-B |
HLA class 1 histocompatibility antigen |
102 |
1.27 |
0.0150 |
40777 |
5.57 |
| 980 |
IPI00004657 |
HLA-B |
HLA class 1 histocompatibility antigen |
121 |
1.34 |
0.0383 |
40777 |
5.57 |
| 981 |
IPI00004657 |
HLA-B |
HLA class 1 histocompatibility antigen |
101 |
1.57 |
0.0008 |
40777 |
5.57 |
| 1080 |
IPI00166729 |
AZGP1 |
Zinc alpha 2 glycoprotein precursor |
147 |
-1.34 |
0.0031 |
34258 |
5.71 |
| 1177 |
IPI00555812 |
GC |
Vitamin D binding protein precursor |
104 |
-1.28 |
0.0151 |
52963 |
5.4 |
| 1186 |
IPI00641737 |
HP |
Haptoglobin |
92 |
-1.29 |
0.0374 |
45205 |
6.13 |
| 1374 |
IPI00978715 |
CLU |
Clusterin |
109 |
-1.25 |
0.0106 |
52495 |
5.88 |
| 1381 |
IPI00410313 |
KIR3KL3 |
Killer cell immunoglobulin like receptor 3DL3 |
132 |
1.25 |
0.0144 |
45470 |
7.27 |
| 1392 |
IPI00022391 |
APCS |
Serum Amyloid P component |
181 |
1.48 |
0.0223 |
25837 |
6.1 |
| 1487 |
IPI00386246 |
AMPH |
Amphiphysin I variant CT3, fragment |
119 |
1.24 |
0.0251 |
31195 |
4.23 |
| 1796 | IPI00007879 | SRF | Serum response factor | 132 | 1.21 | 0.0460 | 51592 | 7.83 |
The 45 identified differential spots found among biological replicates of healed versus dehisced wounds (corresponding to t-test < 0.05 and fold change cutoff > 1.2) were identified via LC-MS/MS and yieled 25 unique protein identifications. The table includes spot number, IPI database number, gene name, protein name, MASCOT identification score, fold change and t-test of expression between healed and dehisced wounds, theoretical molecular weight and pI. *Indicates the 9 proteins selected as markers by discriminate analysis. Spot numbers 332, 336, 548, 763, 978, and 1797 were unable to be confidently identified.