Figure 2.

Boc expression is downregulated in the VTC of Zic2^kd/kd embryos. Frontal cryostat sections from E14.5 Boc^+/lacZ and 16.5 WT and Zic2^kd/kd central retinas reacted with with X-gal histochemistry, hybridized with probes for Boc or immunostained with antibodies against Zic2 and the related Cdon. A, B, The specific expression of Boc in the VTC is particularly abundant in the Zic2-positive domain. C–F, Boc expression is absent in Zic2^kd/kd embryos but maintained in the CMZ where Cdon is also localized. cmz, Ciliary marginal zone; L, lens. Scale bar, 200 μm.