Fig. 3.
Cross-protection against neu-expressing tumors by pE2TM or SKOV3 vaccination. BALB/c mice were immunized with pE2TM or SKOV3 as described. a Anti-neu IgG levels in individual mice were measured 2 weeks after second vaccination by flow cytometry. b Immune splenocytes were collected at the same time. Neu-reactive T cells were enumerated by IFN-γ ELISPOT essay after in vitro stimulation with 3T3/NKB cells. Mice were inoculated s.c. with 2 × 105 D2F2/neu (c) or TUBO (d) tumor cells. There were five to ten mice per group. *P < 0.05, ** P < 0.001 compared to vector-immunized