Fig. 5.

Induction of anti-Her-2 immunity by DNA versus cell vaccines in B6xDR3 Her-2 Tg mice. B6xDR3 Her-2 Tg mice were depleted of CD4⁺CD25^hi Tregs and immunized four times, as in Fig. 4. a Her-2 Ab levels were measured at 2 weeks after the last vaccination. b IgG1 and IgG2c Her-2 Ab induced by DNA and cell vaccines were measured by flow cytometry as described in “Materials and methods”. c At 2 weeks after the last vaccination, mice were inoculated s.c. with 2 × 10⁵ EO771/E2 tumor cells. * P < 0.05 compared to vector or SKOV3 immunized groups