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. Author manuscript; available in PMC: 2013 Nov 14.
Published in final edited form as: Nat Struct Mol Biol. 2013 Apr 28;20(6):10.1038/nsmb.2555. doi: 10.1038/nsmb.2555

Figure 4.

Figure 4

Cysteine blockers inhibit tau-mediated acetyltransferase activity. (a) Tau proteins preincubated with NEM or iodoacetamide (IA), showing minimal acetyltransferase activity. (b) 2N4R-tau preincubated with either increasing concentrations of tubulin (Tub) or preformed microtubules (MTs), showing inhibition of tau acetylation in acetyltransferase activity assays. (c) Acetyltransferase assays of bovine brain-derived microtubule-associated protein (MAP-rich) fractions containing all CNS tau isoforms in the presence of increased tubulin concentrations, followed by immunoblotting with antibodies to Ac-K280 or total tau (T46).