Figure 3. Expression of Fbxo7 rescues parkin mutant phenotypes.

a-b, Overexpression of Fbxo7 suppresses (a) climbing and (b) flight defects of parkin mutants. c, Overexpression of Fbxo7 also suppresses dopaminergic neurodegeneration in the PPL1 cluster of parkin mutants. d-f, (top and middle panels) Toluidine blue stained sections of adult thorax and (bottom panels) TEM images of muscle show that Fbxo7 overexpression suppresses muscle degeneration and mitochondrial disruption in parkin mutants. Toluidine blue scale bars show 200μm (top) and 20μm (middle). TEM scale bars show 2μm. Images are representative of three animals per genotype. g-h, Overexpression of Fbxo7 pathogenic mutants, mt-MTS or isoform 2 by da-GAL4 fails to rescue (g) climbing and (h) flight deficits in parkin mutants. Control genotype is park²⁵/+; da-GAL4/+. Histograms indicate mean ± S.E.M. Significance was determined by one-way ANOVA with Bonferroni correction (*** p < 0.001, ** p < 0.01). For climbing and flight assays at least 50 flies were assessed.