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SEC chromatogram of E5PcF3. Following RPHPLC, active protein E5PcF3 was further separated using a Biosep SEC-S2000 column (300 × 7.8 mm). The mobile phase consisted of 45% acetonitrile containing 0.1% TFA eluted at a flow rate of 1.0 ml/min. Seven peaks eluted from SEC column labelled C1 to C7 were collected and re-evaluated for ACE inhibitory activity.
