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. Author manuscript; available in PMC: 2013 Nov 14.
Published in final edited form as: Exp Biol Med (Maywood). 2009 Aug 5;234(11):10.3181/0811-RM-339. doi: 10.3181/0811-RM-339

Figure 4.

Figure 4

Enhanced HbF synthesis was observed with combined drug treatment. (A) K562 cells were treated with 100 μM L-NMMA), 200 μM L-NAME or 100 μM L-NNA alone or combined with DE or HU for 72 hr. The γGAPD mRNA ratios in UT cells were normalized to one. The bracket and asterisks (* or **) above the graph indicate that all values are significantly different from the corresponding UT values. (B) K562 cells were cultured in DE or HU alone or combined for 72 hr and then mRNA levels were quantified by qPCR and HbF levels by ELISA (see Materials and Methods). The HbF levels were normalized to total hemoglobin and total protein, and levels in UT K562 cells were normalized to one