Skip to main content
. Author manuscript; available in PMC: 2014 Nov 15.
Published in final edited form as: Exp Cell Res. 2013 Sep 19;319(19):10.1016/j.yexcr.2013.09.007. doi: 10.1016/j.yexcr.2013.09.007

Fig 5. BMSCs with attenuated α6 integrin expression proliferate significantly less than control cells expressing the shNT or wild type BMSCs.

Fig 5

A: Representative immunofluorescent images from day 7 3D co-cultures with mCherry-expressing HUVECs cultured on microcarrier beads in the presence of (a) wild type BMSCs tagged with a green cell tracker dye (WT), GFP-expressing (b) BMSCs transduced with non-targeting (NT) shRNA (shNT), (c) BMSCs with silenced α6 integrin (ITGA6-KD), or (d) no BMSCs. B, C: The total DNA extracted from HUVEC/BMSC co-cultures (B) or from BMSC mono-cultures (C) in 3D fibrin gels was assessed as a measure of cell proliferation over a 7 day culture period. *** refers to p ≤ 0.001 relative to the wild-type and shNT controls (n=3).