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Restriction fragment analysis of full-length BAC clone pDEV-vac. A. The patterns corresponded exactly to the predictions based on the complete DEV genome (KF487736), as shown by in silico digests using Vector NTI. B. BAC DNA was isolated and digested with four different enzymes (EcoR V, Bgl II, BamH I, and Xbal I) and separated with a 1.0% agarose gel. The sizes of a molecular weight marker (15,000-bp marker, Takara) are given.
