Table 1.
List of nucleotide sequence different between cloned pDEV-vac [GenBank: KF693236] and the published DEV vaccine sequence [GenBank: KF487736]
|
A. Changes in coding sequences | ||
|
Gene (according to KF487736) |
Modification (nt position) |
Effect |
| UL36 |
A to G (41 128) |
Silent |
| UL36 |
A to C (43 596) |
H to P |
| UL23 |
A to G (75 096) |
I to V |
| UL3 |
C to T (110 795) |
R to Q |
|
B. Changes in non-coding sequences | ||
|
Modification (nt position) |
Comment |
|
| T to C (59 643) |
Between UL30 and UL29 |
|
| Deletion 80 nt : GGGTTCCAAAGGTTTTACGGTA |
|
|
| TTAGGGTTAGGGTTGGCAGGGTTCCAAAGGTTT |
|
|
| TACGGTATTAGGGTTAGGGTTGGCA (119 773–119 852) |
Between LORF2 and IR |
|
| Insertion: AA (129 421–129 422) |
Between IR and US1 |
|
| T to G (143 005) |
Between US7 and US8 |
|
| Insertion: TT (147 986–147 987) |
Between US1 and TR |
|
| Deletion 80 nt: CCTAATACCGTAAAACCTTTGGAACC |
|
|
| CTGCCAACCCTAACCCTAATACCGTAAAACCTTTGG |
|
|
| AACCCTGCCAACCCTAAC (157 498–157 877) |
Between TR and LORF11 |
|
| Insertion: G (158 090) | Between TR and LORF11 | |