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. 2013 Nov 6;10:328. doi: 10.1186/1743-422X-10-328

Table 3.

Primers used in this study

Primer Sequence Domain introduced
18 F
5’-AAAGCTTCGACGAGAGTATCAGCACTCATC-3’
Hind III
18R
5’-AGGATCCTTAATTAACAAGACAGACAAGTATTGCTTGG-3’
BamH I-Pac I
15 F
5’-AGGATCCTTAATTAAGACATTTCCAAGCAATACTTGTCTGTC-3’
BamH I-PacI
15R
5’-AGAATTCAAGCTTATCGACGAACAACTAAGAGTTTGC-3’
EcoR I-Hind III
UL18-s
5’- AAGGACCGCCTACTATCAAA-3’
 
gfp-s
5’- AGAAGAACGGCATCAAGGTG-3’
 
xgpt-s
5’- TCAGTCGGCTTGCGAGTTTA-3’
 
UL15-s 5’-AAACGGGTGGCTGTGCTGAT-3’  

Note. Restriction enzyme recognition sequences are underlined and italicized.