Table 3.
Primer | Sequence | Domain introduced |
---|---|---|
18 F |
5’-AAAGCTTCGACGAGAGTATCAGCACTCATC-3’ |
Hind III |
18R |
5’-AGGATCCTTAATTAACAAGACAGACAAGTATTGCTTGG-3’ |
BamH I-Pac I |
15 F |
5’-AGGATCCTTAATTAAGACATTTCCAAGCAATACTTGTCTGTC-3’ |
BamH I-PacI |
15R |
5’-AGAATTCAAGCTTATCGACGAACAACTAAGAGTTTGC-3’ |
EcoR I-Hind III |
UL18-s |
5’- AAGGACCGCCTACTATCAAA-3’ |
|
gfp-s |
5’- AGAAGAACGGCATCAAGGTG-3’ |
|
xgpt-s |
5’- TCAGTCGGCTTGCGAGTTTA-3’ |
|
UL15-s | 5’-AAACGGGTGGCTGTGCTGAT-3’ |
Note. Restriction enzyme recognition sequences are underlined and italicized.