Table 2.
BLASTN results of 25,417 25-mer oligo sequences
| 25-mers | Hits | Alns | 25/25 | 24/25 | 24/24 | 23/23 | 22/22 | 21/21 | |
|---|---|---|---|---|---|---|---|---|---|
| No hits found |
2,947 |
|
|
|
|
|
|
|
|
| 1 hit (1 aln) |
19,943 |
19,943 |
19,943 |
10,926 |
8,555 |
127 |
116 |
112 |
107 |
| 1 hit (2+ aln) |
1,571 |
1,571 |
4,614 |
2,152 |
2,026 |
161 |
73 |
78 |
124 |
| 2+ hits (1+ aln each hit) |
956 |
2,779 |
19,111 |
7,923 |
9,014 |
389 |
353 |
715 |
717 |
| Total | 25,417 | 24,293 | 43,668 | 21,001 | 19,595 | 677 | 542 | 905 | 948 |
The Clementine reference genome was used as the BLAST database. All the oligo sequences were listed in Additional file 2. Aln – Alignment(s); 1 hit (1 aln) – hit only 1 scaffold with 1 alignment; 1 hit (2+ aln) – hit on only 1 scaffold but with 2 or more alignments, and 2+ hits (1 + aln) – hit on 2 and more scaffolds with one or more alignment to each scaffold.