Figure 2. Allospecific CD8⁺IFN-γ⁺ effector responses predominate over CD4⁺ responses in MHC-mismatched orthotopic lung allografts.

(A) Representative day 10 flow plots of lung allograft or isograft mononuclear cells cultured for 5h in medium alone, in the presence of BALB/c splenocytes (1:1 ratio), or with PMA/ionomycin; gating on H-2^d negative, CD8⁺ T cells in mixed cultures. (B) Cumulative day 10 mean CD8⁺ allospecific IFN-γ responses (expressed as percent of CD8⁺ cells positive for IFN-γ) in allografts versus isografts (n=3–6 mice per group). (C) Representative day 10 flow plots of lung allograft or isograft mononuclear cells with the same culture conditions and analysis as in (A); gating on H-2^d negative, CD4⁺ T cells in mixed cultures. (D) Cumulative day 10 mean CD4⁺ allospecific IFN-γ responses (expressed as percent of CD4⁺ cells positive for IFN-γ) in allografts versus isografts (n=3–6 mice per group). (E) Comparison of day 10 mean CD8⁺ and CD4⁺ allospecific IFN-γ responses in allografts after 5h culture of lung mononuclear cells with BALB/c splenocytes (n=6 mice).