Figure 3. Lung transplant surgery is sufficient for the induction of polyclonal CD4⁺IL-17⁺ T cells.

(A) Representative flow plots of CD4⁺ IL-17 responses at day 10 in lung graft mononuclear cells cultured for 5h in medium alone, in the presence of BALB/c splenocytes (1:1 ratio), or with PMA/ionomycin; gating performed on H-2^d negative, CD4⁺ T cells in mixed cultures. (B) Cumulative day 10 mean CD4⁺IL-17⁺ responses (expressed as percent of CD4⁺ cells positive for IL-17) from isografts versus allografts after culture of lung mononuclear cells in the presence or absence of BALB/c splenocytes (n=3–5 mice per group). (C) Cumulative day 10 mean polyclonal CD4⁺IL-17⁺ responses (expressed as percent of CD4⁺ cells positive for IL-17) from isografts, allografts, and left lungs of littermates receiving no transplant after culture of lung mononuclear cells with PMA/ionomycin.