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. Author manuscript; available in PMC: 2014 May 21.
Published in final edited form as: Anal Chem. 2013 May 2;85(10):10.1021/ac400476w. doi: 10.1021/ac400476w

Figure 2.

Figure 2

Theoretical calculations using amino acid counting. (A) LysC theoretical calculations: 100 000 random yeast LysC peptides were searched against an entire in-silico LysC digest of the yeast proteome with a precursor search tolerance of 20 ppm. The list of resulting peptides was then narrowed if the candidate peptide contained the same number of lysines or lysines and leucines as the peptides used for the search. (B) GluC theoretical calculations: Identical analysis to (A) was performed, but GluC was used as the enzyme for in-silico digest of the yeast proteome.