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. Author manuscript; available in PMC: 2014 May 21.
Published in final edited form as: Anal Chem. 2013 May 2;85(10):10.1021/ac400476w. doi: 10.1021/ac400476w

Figure 6.

Figure 6

Applying lysine and leucine counting to a nanoLC-MS/MS experiment. (A) Sensitivity and median number of candidate sequences considered vs precursor search tolerance. (B) Number of candidate sequences with one match vs precursor search tolerance. The number of these precursors that identified the same peptide as a database search is plotted in red. (C) Lysine and leucine counting applied to all precursors selected in a nanoLC-MS/MS experiment. AAC returned one sequence candidate for 916 precursors, while returning zero sequence candidates for 1358 precursors. (D) High resolution MS1 spectrum of a one match precursor at m/z 620.023. Two peaks spaced 52.4 mTh or 156.9 mDa apart signified the presence of three lysines and 2 leucine residues, mapping this precursor to the peptide GERAKTKDNNLLGKE. (E) An annotated high resolution MS2 belonging to the precursor at m/z 620.023 confirms the peptide GERAKTKDNNLLGKE. Product ions produced by HCD containing lysine or leucine demonstrate the appropriate partners in the MS2 spectrum (insets).