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. Author manuscript; available in PMC: 2013 Nov 14.
Published in final edited form as: Int Immunopharmacol. 2011 Apr 13;11(9):10.1016/j.intimp.2011.03.021. doi: 10.1016/j.intimp.2011.03.021

Fig. 2.

Fig. 2

Decline of the percentages of CD4+ FoxP3+ Treg cells upon in vitro incubation. Either unfractionated splenocytes, positively-selected CD4+ T cells, or negatively-selected CD4+ T cells (CD19-depleted splenocytes) were cultured in parallel with in vitro stimulation. Cells were harvested at the indicated times post in vitro stimulation with plate-bound anti-CD3 and soluble anti-CD28 for anti-CD4 and anti-CD154 staining. FoxP3+ cells were identified using intracellular FoxP3 staining (see Materials and methods). Percentages of CD4+ FoxP3+ Treg cells are representative of three independent experiments.