OT-II TCR Tg+ TCRα-/- mice received multiple doses of OVA protein antigen in vivo (see Materials and Methods). Disease was assessed at 8.5 weeks p.i., with one activational parameter (spleen weight) (A), and one immunodeficiency parameter (splenic mitogenic response to LPS) (B) depicted as representative of all other disease read-outs, which showed the same pattern of results. The results were significant (*, P < 0.05) or not significant (NS, P ≥ 0.05). This experiment has been done twice; the same pattern of result was always obtained.