Table 1. Gene targeting data.
| Strain | Experiment a | Tet | Survivorship b (%) |
Transformed clones |
GFP negative |
Transformation efficiency (×10−6) c | ||
|---|---|---|---|---|---|---|---|---|
| EctopicGFP− | NativeGFP+ | Total | ||||||
| 1 | 1 | No | 5.1 | 4 | 2/4 | 0.39 | 0.39 | 0.79 |
| 1 | 2 | No | 9.3 | 10 | 3/10 | 0.32 | 0.75 | 1.07 |
| 2 | 1 | No | 4.2 | 10 | 6/10 | 1.43 | 0.95 | 2.38 |
| 2 | 2 | No | 8.1 | 12 | 8/12 | 0.99 | 0.49 | 1.48 |
|
| ||||||||
| Mean +/−SD [sum] | 6.7 +/−2.4 | 9 +/−3.5 [36] | 52% +/−16 [19] | 0.78 +/−0.52 | 0.65 +/−0.25 | 1.43 +/−0.7 | ||
|
| ||||||||
| 1 | 1 | Yes | 6.9 | 25 | 12/12 | 3.63 | 0.00 | 3.63 |
| 1 | 2 | Yes | 8.1 | 23 | 10/12 | 2.37 | 0.47 | 2.84 |
| 2 | 1 | Yes | 6.6 | 21 | 7/12 | 1.85 | 1.32 | 3.18 |
| 2 | 2 | Yes | 5.4 | 15 | 8/11 | 2.02 | 0.76 | 2.78 |
|
| ||||||||
| Mean +/−SD [sum] | 6.8 +/−1.1 | 21 +/−4.3 [84] | 79% +/−18 [66 d] | 2.47 +/−0.80 | 0.64 +/−0.55 | 3.11 +/−0.39 | ||
a
Each experiment consisted of five rounds of electroporation, each with 10 μg of donor construct (cut with BamHI/XhoI) and 2×107 cells.
b
Proportion of cells that survive electroporation.
c
Transformed clones / cell that survives electroporation.
d
47 out of 84 clones were screened. This is the adjusted figure.