Fig 1.
Morphine signalling in mouse mesangial cells in vitro. (a) Stimulation of mesangial cells with 1 μM morphine showing a time-dependent increase in phosphorylation of PDGFR-β and MAPK/ERK. (b) Morphine- and 20 ng ml−1 PDGF-BB-induced PDGFR-β and MAPK/ERK phosphorylation are inhibited by 10 μM AG1296, a PDGFR-β inhibitor and naloxone, a non-selective OP antagonist. (c and d) Silencing of MOP and KOP on mesangial cells inhibits morphine- and PDGF-BB-induced MAPK/ERK phosphorylation. (e and f) OP antagonist, MNTX (0.1 μM) and nor-BNI (1 μM), inhibit morphine- and PDGF-BB-induced MAPK/ERK phosphorylation. Mean (sd), n=3.