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. 2013 Nov 14;9(11):e1003337. doi: 10.1371/journal.pcbi.1003337

Figure 4. Effects of the mutation R62A on the global and local backbone dynamics of CheW.

Figure 4

Backbone amide 15N relaxation parameters for CheW vs. residue number are shown. The black squares represent wild-type CheW, the red circles represent R62A mutant, and the green triangles represent the difference between these two constructs. Approximate location of secondary structural elements is shown at the top: (a) the longitudinal relaxation rate R1; (b) the transverse relaxation rate R2; (c) 1H-15N NOE; (d) the extracted order parameter S2 (e) the differences phenomenological transverse relaxation rate constant ΔRex = Rex(20 ms)−Rex(1 ms); (f) the differences between the ΔRex in (e) ΔRex(R62A)−ΔRex(WT).