Figure 5. Correlation of amino acid pair propensities between natural and designed covarying pairs.

A) Heat maps of amino acid pair propensities at pairs of positions that are highly covarying in both designed and natural sequences. Red pairs are over-represented at covarying positions and blue pairs are under-represented at covarying positions. The values are shown as Z-scores, which denote the number of standard deviations above or below the mean. B) Correlation of amino acid pair propensity Z-scores between designed and natural sequences. The left plot shows the correlation from flexible backbone design sequences and the right plot shows the correlation from fixed backbone design sequences. A Pearson correlation coefficient (r) is shown for each plot.