Figure 9. Sequence homology clustering of secreted proteins in M. haptotylum.

Clusters of secreted proteins (nodes) containing at least three protein members are shown and Pfam domains in the clusters are indicated. The two rings indicate a cluster containing two groups of proteins with different Pfam domains and joined by only one protein. Small secreted proteins, SSPs (<300 amino acids) are colored: Blue nodes indicates proteins belonging to the core set; green are lineage-specific proteins and red nodes are species-specific proteins. Grey nodes are secreted proteins with a length of 300 amino acids or longer. Five red stars indicate SSPs that are more than 10 fold upregulated during infection versus knobs and belong to the 10% most highly expressed genes during infection. O indicates orphan sequences lacking matches to the Pfam database and other species in NCBI nr database. Blue Δ indicates core genes and green Δ indicates lineage-specific genes with 10 fold differential gene expression pattern between orthologs of M. haptotylum and A. oligospora during infection. Cluster 1 contains the following Pfam domains: Abhydrolase_2, Adeno_E1B_55K, Alpha_GJ, ArabFuran-catal, ASF1_hist_chap, BBE, CBM_1, Cellulase, Cutinase, CVNH, DUF1023, DUF1191, DUF1680, DUF291, End_N_terminal, Esterase, Esterase_phd, FAD_binding_4, Glyco_hydro_6, 7, 10, 11, 12, 16, 18, 26, 28, 30, 43, 45, 61, 62, 72, GMC_oxred_C, GMC_oxred_N, HC2, Herpes_gE, Kei1, Lipase_GDSL, LysM, Melibiase, Muc_lac_enz, Mucin, OmpW, Pec_lyase_C, Pectate_lyase, Pectinesterase, Peptidase_C8, Podoplanin, Pyr_redox, Pyr_redox_2, RE_AccI, Ricin_B_lectin, Sec10, SKN1, Stig1, Syndecan, Tyrosinase, X8 and Yip1.