Table 2. Mutations identified in 15 starved, rifampicin resistant isolates.
Location on the chromosome | Gene | Number of reads1 | Nucleotide change | Number of 15 strains carrying mutation2 | Position in protein | Amino acid change |
4179710 | rpoB | 380/838 | A to T | 6 | 148 | Gln to Leu |
4180801 | rpoB | 131/888 | T to C | 2 | 512 | Ser to Pro |
4180802 | rpoB | 22/871 | C to A | 1 | 512 | Ser to Tyr |
4180813 | rpoB | 19/856 | G to A | 1 | 516 | Asp to Asn |
4180843 | rpoB | 106/917 | C to T | 3 | 526 | His to Tyr |
4180845 | rpoB | 95/909 | C to A | 1 | 526 | His to Gln |
4180954 | rpoB | 110/1015 | A to C | 1 | 563 | Thr to Pro |
Number of reads at which variant allele was called out of total number of reads at that position.
Based on PCR and Sanger re-sequencing from individual strains.