Figure 10. DUSP1 is involved in the regulation of adaptive immune response during VACV infection.

A) Magnitude of E3-specific CD8 T cell response after intraperitoneal (i.p.) inoculation with either WR (10⁷ PFU/mouse), MVA (2×10⁷ PFU/mouse) or NYVAC (2×10⁷ PFU/mouse). The values represent the sum of the percentages of CD8⁺ T cells secreting IL-2 and/or TNF-α and/or IFN-γ. CD8⁺ T cell responses were measured 10 days after inoculation by ICS assay following stimulation of splenocytes with E3 peptide. All data are background subtracted. B) Magnitude of E3-specific CD8 T cell response after intranasal (i.n) inoculation with either WR (10⁵ PFU/mouse), MVA (5×10⁷ PFU/mouse) or NYVAC (5×10⁷ PFU/mouse). C) Functional profile of E3-specific CD8 T cell responses after i.p inoculation as in A). All the possible combinations of the responses are shown on the x axis and the percentage of the functionally distinct cell populations within the total CD8 T cell populations are shown on the y axis. Responses are grouped and color-coded on the basis of the number of functions. D) Functional profile of E3-specific CD8⁺ T cell responses after i.n. as in B). Results are represented as in C). Graphs show a representative example of three independent experiments performed. p<0.05 (*), p<0.005 (**) and p<0.001 (***).