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. 2013 Nov 14;8(11):e79824. doi: 10.1371/journal.pone.0079824

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© 2013 Mill et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) 20% ethanol consumption in F344 rats trained using the IAE procedure before and after a two-week deprivation period. (B) Animals in the high group exhibited a significant escalation in ethanol intake following a two week deprivation period. (C) Ethanol preference before and after a two-week deprivation period. (D) Animals in the high group exhibited a significant increase in ethanol preference following deprivation. The values are expressed as mean ethanol intake (g/kg/24h) or preference (ratio of ethanol over total fluid intake) ± SEM. To calculate the ADE effect the five drinking sessions preceding and three drinking sessions following deprivation were analyzed by one-way ANOVA, **, p<0.01 comparing pre- and post-deprivation periods.