Skip to main content
. 2013 Nov 14;8(11):e80181. doi: 10.1371/journal.pone.0080181

Figure 1. Semi-quantitative analysis of Pmca4a, b mRNA via RT-PCR in the murine female reproductive tract.

Figure 1

A) The 469 bp PCR product represents Pmca4a, and that of 278 bp corresponds to Pmca4b. Murine testis (T) cDNA served as positive control. Negative control was performed in the absence of reverse transcriptase (-RT). Gapdh mRNA was used as an internal control (B). Pronounced expression of Pmca4b was detected in the uterus, whereas PMCA4a showed more prominent expression in the oviduct than in the testis. A 100 bp ladder was run in the left lane of each panel. O, oviduct; U, uterus, and V, vagina.