Figure 4. Indirect Immunofluorescence of PMCA4a in the murine myometrium of the uterus during the estrous cycle.

In addition to the endometrium, the muscles and mesothelium of the myometrium (M*, M, respectively) were positively stained for PMCA4a. Elevated levels of PMCA4a immunoreactivity were detected at the boundaries of the epithelial cells lining the pro-estrus uterine glands (g). The nuclei were visualized by staining with Draq-5 (blue). Negative controls (NC) of diestrus, pro-estrus, estrus, and metestrus phases are respectively shown (d, h, l, and p). The images were captured using confocal microscopy and a 20x (a plan-Apochromatic) objective lens. GE = glandular epithelium; L = lumen; ST= stroma. Bar = 100 µm (same scale for all micrographs).