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. 2013 Nov 14;8(11):e80117. doi: 10.1371/journal.pone.0080117

Figure 4. Treatment of a mixture of Chlamydomonas reinhardtii and Mycrocystis aeruginosa with hypochlorite during 20 minutes.

Figure 4

(A) FCM density plot distinguishing the two populations in the starting mixture based on orange (ex. 488 nm/em. 585 nm) and red auto-fluorescence (ex. 488 nm/em. 670 nm). (B) Raw RT-FCM data of red auto-fluorescence intensity, resulting from the excitation of chlorophyll. Hypochlorite was added after 5 minutes (dotted line) (C) Raw RT-FCM data of orange auto-fluorescence intensity. (D) Processed data (30-second intervals) showing stable cell concentrations for both clusters during the experiment. (E) Changes in the red and orange fluorescence intensities of C. reinhardtii. (F) Changes in the red and orange fluorescence intensities of M. aeruginosa.