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WA09 human ES cells and Ainv15 mouse ES cells were cultured for three days on feeder free conditions in the indicated medium (untreated cells, C; EDA+/+ MEF-CM, CM; EDA+ including peptide, PEP). RNA was extracted and the expression of Oct4, Sox2 and Nanog pluripotency gene markers was analyzed by RT-PCR. The expression of the housekeeping GAPDH gene was used as control.
