. 2013 Nov 14;8(11):e80472. doi: 10.1371/journal.pone.0080472

Table 2. Effects of regimen 2 restraint stress on attachment reaction and blastocyst hatching.

Time for examination	Treatment	Mice showing plugs^*	% Mice with IS^$	IS per mouse	Blastocysts per mouse	Hatched blastocyst/mouse	% Hatched/total blastocysts^*
D4 20:00	Control	12	83.3±16.7^a	6.4±1.0^a	10.9±0.7^a	0.7±0.3^a	6.8±1.1(9/131)^a
	Stressed	12	16.7±8.3^b	1.6±1.1^b	9.6±0.9^a	0.4±0.2^a	4.8±2.6(5/95)^a
D4 24:00	Control	12	100±0.0^a	8.9±0.7^a	6.8±0.8^a	3.1±0.5^a	41.9±4.9(34/82)^a
	Stressed	10	61.1±5.6^b	4.5±1.3^b	6.7±1.6^a	1.0±0.7^b	18.3±9.8(8/67)^b
D5 08:30	Control	15	100±0.0^a	11.7±0.8^a	4.1±0.9^a	5.5±0.7^a	96.9±1.6(60/62)^a
	Stressed	15	73.3±6.7^a	8.1±1.5^a	5.5±1.2^a	5.0±1.3^a	63.5±11.0(56/88)^b

^a–b

: Values without a common letter in their superscripts differ (P<0.05) in the same column within time points.

*Each treatment was repeated 3 times with each replicate containing 3–5 mice.

% Mice with IS indicates the ratio of mice with implantation sites (IS)/mice showing vaginal plugs, and Independent samples t-tests were conducted using SPSS to locate the difference between stressed and control mice.